| Summary: | 碩士 === 臺北醫學大學 === 醫學科學研究所 === 96 === “Koso” is a healthy supplement extracted from 100 kinds of vegetables and fruits fermented by yeast and the lactic acid bacteria for two years. After “Koso” extraction, numerous fermented plant fibers were discarded. In order to reuse these fermented plant fibers efficiently, the aim of this study was to explore whether they may improve the host’ s gut microflora and immunity by in vitro and in vivo（human and animal ）experiments.
Comparing to the well-known prebiotics fructo-oligosaccharide or inulin, the fermented plant fibers had better prebiotic function to lactobacilli than bifidobacteria by in vitro assay.
Seventeen healthy volunteers (age: 18~35 years) were recruited into this free-living and self control study. The 10-week trial comprised an initial lead-in week, a 3-week treatment I phase (taking fermented plant fibers alone, ten tablets once a day), a 2-week washout phase, a 3-week treatment II phase (taking fermented plant fiber with L. rhamnosus GG), and followed by a final week of washout. After oral administration of the fermented plant fibers for 3 weeks, the fecal lactobacilli and bifidobacteria were increased efficiently. However, the decrease of fecal harmful bacteria, Clostridium perfringens in fermented plant fibers group was inferior to that of the synbiotic group. In this study we demonstrated that consumption both of the fermented plant fiber and L. rhamnosus GG may have the best effect on our intestinal health. However, the harmful bacteria may reappear in our intestine after stopping administration of the symbiotics.
Twenty four BALB/c mice were divided into two groups (prebiotics and synbiotics groups). Referring to the human dosage, each mouse was daily fed with 400mg fermented plant fiber or fermented plant fiber with L. rhamnosus GG for 8 weeks. In this animal study, we also proved that taking fermented plant fibers alone or fermented plant fibers with L. rhamnosus GG for two weeks could increase the fecal lactobacilli and bifidobacteria, while decrease the fecal coliform bacteria. However the increase of fecal beneficial bacteria may reach to a plateau after 5 weeks, even the feeding period was extended to eight weeks. For investigating the immunomodulating effect of the fermented plant fibers on BALB/c mice, blood samples were drawn from the retroorbital sinus every two weeks, and the mice were sacrificed on day 56. The phagocytosis of the neutrophils and monocytes in the synbiotic groups were significantly better than those of the prebiotics group. These results might be correlated with the increasing of L. rhamnosus GG in the intestine of the synbiotic groups. Compared the splenocyte cytokine secretion, the IFN-γ secretion increased, while the IL-10 secretion decreased in the sybiotic group. The result was agreed with previous study that the immunomodulating activity of L. rhamnosus GG was through upregulation of Th1 function.
Conclusion: The fermented plant fibers do have prebiotic effect which may stimulate the proliferation of fecal beneficial bacteria while inhibit the growth of fecal harmful bacteria. Therefore, we further added L. rhamnosus GG to these fermented plant fibers residues to develop a novel “synbiotic” which had better prebiotic effect than the fermented plant fibers alone.
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