Early detection of latent tuberculosis using super-paramagnetic iron oxide nanoparticle enhanced functional magnetic resonance imaging

• Main Authors: Sheng-Hung Hsieh, 謝昇宏
• Other Authors: 賴文福
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/53586642404042141561

碩士 === 臺北醫學大學 === 醫學科學研究所 === 96 === Tuberculosis is one of the threatening human diseases anciently from today, and also the one of the top three global infective disease killers. Since mid 1990, originally thought the TB would soon be consigned to the pages of history gradually has sign reviving, and no matter the incidence or mortality all have the tendency to increase year by year. One-third of the population is infected with the tuberculosis in the world now, and there are 2-3 million people die of this disease every year. In order to effective removing this threat, except the often protect the health and sufficient nutrition all the times, to accurate diagnosis and effective treatment is one of the important methods to eliminate the tuberculosis. The main purpose of this research is trying to provide a kind of new tuberculosis diagnosis method. According to the molecular imaging character, it can in vivo characterization and measurement of biologic processes at the cellular and molecular level. We use the noninvasive in vivo magnetic resonance imaging 2 as a detection instrument combine with Mycobacterium tuberculosis specific probe SPIO-Tb-Ab (super-paramagnetic iron oxide-tuberculosis-antibody), catching and strengthening the image at the same time. We hope this diagnosis method cans being used for detection latent tuberculosis and extra-pulmonary tuberculosis in early days. We planted the attenuated vaccine BCG (Bacille Calmette-Guerin) on mouse back by subcutaneous injection and then intravenous injection with SPIO-TB-Ab for 3.0T MR imaging. We found there is a MRI signal in the injection site and the results is coincident with the tissue section stain. According to the results, we think this method of utilizing the idea of the molecule image to combine MRI instrument perhaps can offer a kind of new diagnosis method to tuberculosis.