Studies of molecular mechanisms of NQ-1 on the hypoglycemic effect in vitro and in vivo

• Main Authors: Nai-Qi Chen, 陳乃琦
• Other Authors: 梁有志
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/33095871920463522798

碩士 === 臺北醫學大學 === 醫學科學研究所 === 96 === Peroxisome proliferator-activatived receptors (PPARs) are members of the nuclear hormone receptor superfamily of ligand-dependent transcription factors. PPARs play a central role in insulin sensitivity, lipid metabolism, glucose homeostasis, cell cycle progression, and inflammation. For the control blood glucose level of diabetes mellitus patient, searching PPAR activators from natural and synthetic chemicals, and discovering their associated functions is one of the most important works in the field. NQ-1 is a hydrophobic chemical and extracted from Chinese herb medicine. First, NQ-1 significantly activated both of PPARa and PPARg in a dose-dependent manner by transition transfection assay. Second, NQ-1 markedly induced the expression of PPARa and PPARg in HepG2 cells and 3T3-L1 respectively. In addition, NQ-1 significantly induced the expression of adipogenic factors such as aP2, LPL, C/EBPa and fatty acid synthase in 3T3-L1cells. Third, NQ-1 also induced the expression of PPARa target genes such as CPT-1 and ACS in HepG2 cells. Finally, obese diabetic mice (db/db mice) were treated with NQ-1, and found that NQ-1 markedly reduced plasma glucose level, however did not change the plasma insulin level and serum triglyceride level. These results suggest that NQ-1 is a dual PPARa/g activator and may have a beneficial effect on the therapy of typeⅡdiabetes mellitus.