Studies on chemical constituents and anti-inflammatory activity of cultivated Pycnoporus sanguineus

• Main Authors: Hsueh-O Lin, 林雪娥
• Other Authors: 徐鳳麟
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/41935893820524796823

碩士 === 臺北醫學大學 === 生藥學研究所 === 96 === Pycnoporus sanguineus (Fr.) Murr is a slow-growing saprophytic fungus which causes decay of certain types of wood in the forests of tropical and subtropical areas. Because of the material is limited on the island and poor growth rate in nature, liguid culture of the fungus was attempted. The results showed that the fungus was cultured for 49 days to reach the maximal dry weight of 16.13±2.10 g/l. The yield of polysaccharides (PS) of P. sanguineus for 21 days was 1.02±0.40 g/l. The yield of ethanolic extract of mycelial of P. sanguineus for 28 days was 6.10±1.90 g/l. Compositional analysis PS of P. sanguineus showed that fucose, glucose and mannose were the predominant sugars in the value of 64.81±0.15, 18.70±0.05 and 17.56±0.03 μmol/g PS, respectively. Bioassay-guided fractionation was led to the isolation of four ergostane skeleton type steroids, including ergosta-7,22-dien-3β-ol (ST-1: 1), 3β,5α-dihydroxyergosta-7,22-dien-6-one (ST-2: 2), 3β,5α,9α-trihydroxyergosta-7,22-dien-6-one (ST-3: 3), and 3β,5α,6α-trihydroxyergosta-7,22 -diene (ST-4: 4) 。All were known compounds. From the isolates, ergosta-7,22-dien-3β-ol was isolated from P. sanguineus previously. The others were the first isolation from mycelial of P. sanguineus. Their structures were determined by 1H, 13C, 2D NMR, and MS spectral analyses. The bioassay-guided fractionation of the crude ethanolic extract showed significant inhibition of LPS induced NO production in BV-2 cells up to a concentraction of 50 μg/ml for 87.5 %. EA-layer extract was also showed significant inhibition of LPS induced NO production in BV-2 cells up to a concentraction of 20 μg/ml for 87.5 %. BuOH-layer extract of 20 μg/ml for inhibition of 45.5 %. Compound ST-1 of the inhibition at the concentraction of 15 μM in the value of 43.5 %.