Biodegradability and Biocompatibility of the Scaffold Derived from Cell Wall Component of Rhizopus stolonifer

• Main Authors: Yao-Zheng Huang, 黃耀正
• Other Authors: Ching-Hua Su
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/75843694163977817339

碩士 === 臺北醫學大學 === 醫學研究所 === 96 === In the present study, the sporangia-formation-delayed mutant strain Rhizopus stolonifer F6 was used to obtain mycelium mattress by liquid culture. The mycelium mattress was treated with 1N NaOH at 121oC for 20 minutes and followed by lyophilization to have RHIZOCHITIN (RC). RC was then again washed with 95% alcohol to remove non-saponification lipid to get alcohol-treated RHIZOCHITIN (ARC). RC and ARC together with a commercial product BESCHITIN-W (BC) for positive control were employed as scaffold for the tests of biocompatibility and biodegradability in vitro and in vivo. In vitro tests included lysozyme degradation and co-culture to fibroblast cell line (L-929) with the weight change and microscopy (light and scanning electron microscopy). Lysozyme did not digest RC and ARC at the first three weeks but significant degradation was observed in the group of BC by the decrease in weight and the observation under SEM. RC and ARC did not affect the survival of fibroblast cell line in culture condition with an obvious decrease in weight. However BC significantly decreased the survival rate of the fibroblast cell with only slightly degradation of the scaffold. In vivo study demonstrated that RC and ARC had totally degraded after 2 weeks imbedded in subcutaneous layer of SD rats and BC showed a pattern of slow degradation through a four-week observation. No allergic reaction or irritation on the skin of the rats was observed through the experiment for RC ARC and BC. Finally, the tissues of the embedded area were sampled by a punch of 8 mm in diameter after 4 weeks after imbedding of the scaffolds and section of the tissues revealed that RC and ARC were far more degradable than BC in both HE and PAS stains. The study suggested that the cell wall component derived from Rhizopus stolonifer F6 was biocompatible and biodegradable.