Magnolol suppresses HER2/neu expression and its mediated Metastasis

• Main Authors: Yi-Ting Cheng, 鄭逸婷
• Other Authors: Tzu-Chao Chuang
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/70553839317807103346

碩士 === 淡江大學 === 化學學系碩士班 === 96 === The oncoprotein HER2/neu is know to be overexpressed in several human cancers and it is associated with increased metastasis and poor prognosis. Magnolol, an active component extracted from Magnolia officinalis, has shown several pharmacological activities such as anti-tumor, anti-oxidant and anti-inflammatory effects. However, the effects of magnolol on the HER2/neu-overexpressing cancer cells are unknown. Here, we demonstrated that magnolol induced HER2/neu-overexpressing cancer cell death, in a dose- and time-dependent manner. We also reveled that magnolol could suppress the HER2/neu gene expression. We also elucidate that EGF specifically restores the activation of MMP2 after its activity was reduced by magnolol. Furthermore, LY294002, a PI3K inhibitor, attenuated the restoration of MMP2 activity by EGF. These finding suggest that magnolol can suppress HER2/neu expression, and then inhibiting the PI3K/Akt-signaling pathway to attenuate the p185HER2/neu mediated metastasis. Taken together, we found that magnolol is potent in suppressing metastasis-associated properties of HER2/neu-overexpressing ovarian cancer cells.