| Summary: | 碩士 === 國立臺北科技大學 === 光電工程系研究所 === 96 === We construct a new Laser Capture Microdissection (LCM) system by Using Electric Moving Stage. It spends a lot of time in positioning when the new LCM system with near-field fiber probe is integrated with microscopy. For overcoming the shortcoming, we designed a precision stage for moving the sample to adjust the target to be in the center of the scope. The fiber probe was set over the view center, and allowed to move only up and down. The designed stage includes a stepping motor and piezo actuators for moving fine in X-Y directions. We build a component of Human Machine Interface on electric moving stage control system and LCM system by Visual Basic.
Then, we actually use electric moving stage on LCM experiments. By statistical analysis, we obtained the average displacement value of the stage is 3.265 μm and Relative standard deviation(RSD) is 0.00025. We successfully melted the EVA transfer film into figure by using the 70 nm fiber probe. The target can be moved to the center of scope in one minute by this new stage, then the process of laser capture be completed more quickly and higher precision control.
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