| Summary: | 碩士 === 國立臺北科技大學 === 化學工程研究所 === 96 === Electromagnetic Field (EMF) is shown to be able to accelerate bone healing and improve on osteoporosis. However the healing effect of EMF on large size bone fracture(＞1cm) is not significant. Tissue engineering uses biocompatible porous scaffolds to fill up large defects, promote cell in-growth and can help reduce bone fracture healing time.
In this study we will explore effects of EMF on cells growing on tissue engineering scaffolds. We cultivated osteoblast (7F2) on chitosan scaffold and chitosan film, and subjected the cells to EMF (Intensity 18-30 Gauss, 75Hz, impulse width 1.3 ms, induced electrical amplitude 3.5mv) for 2 hours each day for three weeks. On week 1、week 2 and week 3, tested cell viability(MTT assay), alkaline phosphatase activity(ALP), total protein, calcium content, DNA content, cell morphology (SEM、fluorescent stain) and calcium stain. For chitosan we subjected material to EMF for 2 hours for three weeks and examined its compression strength, degradation rate and contact angle.
We observed increased cell viability, proliferation, calcium deposition and DNA content in cells exposed to EMF; ALP activity decreased by week 3 in cells exposed to EMF. From SEM and fluorescent stain pictures, we observed osteoblast typical and cell morphology on chitosan scaffold(Polygonal and Spindle-like shape) after EMF exposure. For chitosan, we found there is no difference in compression strength, degradation rate and contact angle after EMF exposure.
In conclusion, our results show EMF promote osteoblast proliferation and differentiation on chitosan scaffold. For mimic the environment of human bone tissue, it is necessary to do in vivo tests in the future for investigating the effect of EMF on tissue engineering.
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