| Summary: | 碩士 === 東海大學 === 畜產與生物科技學系 === 96 === The purpose of this study was to investigate the effect of the velvet antler of Formosan sika deer on antioxidative ability in senescence-accelerated mice (SAMP8). Six-month-old SAM P8 mice were fed commercial chow containing 0.2% antler powder (antler group) or control chow for 8 week, and 4 males and 4 females from each group were sacrificed at week 2, 4, 6 and 8, respectively. The hydrogen peroxide (H2O2) content and the activities of antioxidative enzymes, superoxide dismutase (SOD), cellular glutathione peroxidase (cGPX) and catalase (CAT), in plasma, liver and brain were analyzed. In male, the plasma H2O2 content at week 4 was significantly lower in antler group; the SOD activities at weeks 2, 8 and 2-8, and the plasma cGPX activity at week 2 were significantly lower in antler group. In female, the plasma H2O2 content was not different between groups; the SOD activities at weeks 4, 8 and 2-8 were significantly lower in antler group; the cGPX activities were not different between groups. In male, the liver H2O2 content at week 8 was significantly higher in antler group; the liver SOD activity at week 4 was significantly lower, but higher at weeks 6 and 8 in antler group; the liver cGPX activities at weeks 6 and 8 were significantly higher in antler group; the liver CAT activity at week 4 was significantly lower but were higher at weeks 6 and 8 in the antler group. In female, the liver H2O2 contents at weeks 2 and 4 were significantly lower in antler group; the liver SOD activities were not different between groups; the liver cGPX activity at week 4 was significantly lower in antler group; the liver CAT activity at week 8 was significantly lower in the antler group. In male, the brain H2O2 content was not different between groups; the brain SOD activity at week 6 was significantly lower in the antler group; the brain cGPX activity was not different between groups. In female, the brain H2O2 content at week 2 was significantly higher in the antler group but lower at 6 week; the brain SOD activity at week 6 and the brain cGPX activity at week 4 were significantly higher in antler group. Total RNAs were extracted from liver and brain, and the reverse transcription was performed to synthesize cDNAs. The TaqMan system was used for real-time polymerase chain reaction analysis to detect the gene expression of the three antioxidant enzymes. The results indicated that there was no difference in gene expression of antioxidative enzymes in liver between groups. In brain, the SOD mRNA expression in the antler group at week 8 was significantly lower than in the control group. The cGPX and CAT mRNA expression in the antler group at week 4 was significantly higher than in the control group. Based on the results in this study, addition of Formosan sika deer antler to the diet didn’t affect the activities of antioxidant enzymes and H2O2 content in plasma. In liver, the activities of antioxidant enzymes increased in male and H2O2 content decreased in female. In brain, there was no effect on the activities of antioxidant enzymes and H2O2 content. On the genes expression, there was no difference for the gene expression of those genes in liver between groups. In brain, cGPX and CAT mRNA expressions were higher in the week 4 but SOD mRNA expression was lower in the week 8. Therefore, Formosan sika deer antler can not increase the antioxidative ability in SAMP8 mice.
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