Association of Host Genetic Polymorphisms and Helicobacter pylori-induced Disease Outcome

• Main Authors: Chih-Shin Chang, 張稚鑫
• Other Authors: 林念璁
• Format: Others
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/8d8zs7

碩士 === 慈濟大學 === 微免暨分子醫學研究所 === 96 === Helicobacter pylori infection is closely associated with gastroduodenal diseases. It has become apparent that not only the characteristics of the pathogen but also host genetics play an important role in determining susceptibility to and severity of infections. In this study, we evaluate the relationship between host genetic polymorphisms and disease outcome in H. pylori-infected aboriginal and nonaboriginal populations in eastern Taiwan. We also examine human β-defensins expression in gastric mucosal tissues with or without H. pylori infection for better understanding the innate immune response to H. pylori. In the experiment, 191 H. pylori-infected patients (97 aborigines and 94 nonaborigines) and 171 H. pylori-negative controls (90 aborigines and 81 nonaborigines) are enrolled in the study. Polymerase chain reaction (PCR)-restriction fragment length polymorphism analysis was performed for the K469E variant of the ICAM-I gene and the E266K variant of the NOD1 gene. We used reverse transcription- polymerase chain reaction and immunohistochemistry to examine human β-defensins expression in gastric mucosal tissues. In our results, there was a significant difference in ICAM-1 K469E polymorphism between aborigines and nonaborigines (p<0.001). In addition, KK genotype of ICAM-1 K469E polymorphism and AA genotype of NOD1 E266K polymorphism may be a potential risk factor in disease outcome. The mRNA expression of human β-defensin 2 in H. pylori-positive patients was significantly higher than that in H. pylori-negative patients (p<0.05).