Evaluation of Differentially Expressed Genes of Neocaridina denticulata Exposed to the Nonylphenol as Biomarkers under Laboratory Conditions

• Main Authors: Chang-lun Liu, 劉昌倫
• Other Authors: Hung-hung Sung
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/2ak54h

碩士 === 東吳大學 === 微生物學系 === 96 === Nonylphenol is a metabolic product from the microbial degradation of detergents. Because of disrupting function of normal endocrine and physiological activity, NP is regarded as an endocrine disrupting chemical（EDC）. NP exerts its effect through the trophic chain and bioaccumulation in human. In this study, genomics signals were used to assess toxicity of NP in Neocaridina denticulata. We identified differentially expression genes of Neocaridina denticulata exposed to NP by suppression subtractive hybridization（SSH）. Neocaridina denticulata treated with 0.5 mg/L NP for 1 day, 19 expressed sequence tags （ESTs） were obtained in forward subtracted library and 46 ESTs in reverse subtracted library. When exposed in 0.5 mg/L NP for 7 days, we obtained 33 ESTs in forward subtracted library and 34 ESTs in reverse subtracted library. 14 ESTs recognized up-regulated or down-regulated genes in NP-related gene library and gestation-related library have been reported to be associated with immunity（NP1-2）, stress（rNP1-10）, development（NP1-9）, metabolism（rNP1-8）, translation factor（NP1-7）,and unknown（NP1-17, rNP1-16, rNP1-17, rNP1-19, rNP1-20, rNP1-21, rNP1-22,and NP1-27）. Evaluating the sensitivity of each gene for NP, a change of 4 genes, including rNP1-10、 rNP1-17、rNP1-20、rNP1-27, was detected after treatment with 0.001, 0.01, and 0.5 mg/L for one day. A change of gene NP1-9 was detected after treatment with 0.01 and 0.5 mg/L of NP. The level of the other genes expression was different after treatment with 0.5 mg/L of NP. It suggests that differentially expression genes pattern can be used as a model for detecting toxicity of NP.