Functional Studies of Lvdou huang and Phellinus linteus

• Main Authors: Yu-Chun Wang, 王昱鈞
• Other Authors: Hui-Yu Huang
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/27640236933232639927

碩士 === 實踐大學 === 食品營養與保健生技研究所 === 96 === The Chinese herbs medicine is a used to treat the disease in several millenniums, and it acts the important role in the traditional therapy. In these years, many studies demonstrate that Chinese herbs have many pharmacology effects. The aim of this study divided into two categories. First, try to study the effect of Lvdou huang (LH) in antioxidant activity, whitening effect in vitro and energy metabolism in vivo. The methanol and ethanol extraction of LH were used to evaluated the free radical scavenging rate and the ability of tyrosinase inhibition rate. The MTT assay, SOD, and catalase activity were examined with MDCK and A375 cell. The results indicated that 4000 μg/ml LH extract was equivalent to 25 μg/ml Vitamin C in DPPH scavenging rate. The ability of tyrosinase inhibition of ethanol LH extract showed the better result than methanol extract, and the 4000 μg/ml ethanol LH extract was 44.1% higher than the 250 μg/ml Vitamin C. The LH extract didn’t influence survival rate of MDCK and A375 cell, but was significantly increased proliferation of MDCK cell. In addition, the ethanol LH extract increased antioxidant enzymes activity in MDCK cell significantly, especially at 250 μg/ml ethanol LH extract. In animal model, the BALB/c mice were oral administered sterile water or LH at different dosages, including 25 mg/day, 50 mg/day, or 100 mg/day, respectively for 4 weeks. We collected the mice liver to analysis SOD, catalase, and GPx activity, and the mice blood to analysis plasma glucose, T-Cho, TG, HDL-c and LDL-c. The results indicated that medium dose LH group significantly increased liver SOD and catalase, and high dose LH group significantly increased liver GPx activity. All LH groups were significantly lowering the plasma glucose, but didn’t change serum HDL-c and LDL-c. Serum T-Cho of medium and high dose LH groups were significantly lower than control group. Serum TG of high dose LH group was significantly lower than control group. In conclusion, LH exhibited many beneficial effects in antioxidant activity, whitening effect, and energy metabolism.Second, the hepato-protective effect and energy metabolism of Phellinus linteus (PL) in hepatoma SCID mice were be studied. The 4 weeks old male SCID/CB-17 mice were divided into 5 groups, and were bearing with Hep3B cells or without. The mice were oral administered sterile water (with or without hepatoma SCID mice) or PL at different dosages (with hepatoma SCID mice), including 50 mg/kg/day, 100 mg/kg/day, or 200 mg/kg/day, respectively for 8 weeks. The SCID mice were sacrificed and were collected their blood and liver. The body weight, serum GOP, GPT, T-Cho, TG, HDL-c, LDL-c, liver SOD and catalase were determined to assess liver protection activity and energy metabolism rate. The results indicated that oral administered low dose PL group decreased GOT and GPT, and increased liver SOD and catalase. However, medium and high dose PL groups were against the results with the low dose. All PL groups were significantly increasing the plasma glucose, and decreased serum insulin with low dose PL group. However, the level of plasma T-Cho, TG, HDL-c and LDL-c didn’t change in all PL groups. Low dose PL group also increased total muscle protein, but muscle glycogen didn’t be influenced. In conclusion, low dose PL exhibited beneficial effects of hepato-protection. In addition, PL may improved to lower blood glucose, and to reduce muscle protein catabolism inducing by hepatoma.