| Summary: | 碩士 === 靜宜大學 === 應用化學研究所 === 96 === In this study, berberine nano-encapsulations were prepared by phase inversion temperature (PIT) emulsification method, sodium alginate was used as a carrier. The particle structure of nano-encapsulations was investigated by transmission electron microscope (TEM), particle size was measured by Nano-sizer distribution meter. In vitro drug released study of berberine nano-encapsulations was analyzed using simulated gastric fluid (SGF) and simulated intestinal fluid (SIF). The fluid of sodium alginate through the gastrointestinal tract (GIT) was traced by fluorescence image detector (Kodak 4000). In vivo drug released study was performed by section of intestinal samples of nano-particle-fed Balb/c mice, the fluorescent signal was detected by Fluorescence reader. The results showed that the prepared berberine nano-encapsulated particles exhibited a spherical structure. Particles were grown up with increasing the concentration of cross-linker and sodium alginate, the particle size distributed in the range 77-181 nm. With increasing the concentration of cross-linker and the ratio of carrier and drug, the entrapment efficiency was increased. The entrapment efficiency was obtained in the range 18-46%. In vitro drug releasing study indicated that berberine nano-encapsultions were able to prolong the drug releasing. Additionally, in vivo study showed that the bioadhesive property of sodium alginate could prolong the drug retention in the mouse GIT. Taken together, our observations suggest that PIT-prepared berberine nano-encapsulations might be effectively applied in drug delivery.
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