| Summary: | 碩士 === 靜宜大學 === 食品營養研究所 === 96 === This study was to investigate the relationships between the antioxidation and human Caco-2 colon adenocarcinoma cell inhibition of food polysaccharide gums, their methylated derivatives and hydrolysates, in order to conclude the food polysaccharides of best bioactivities. The samples examined included: pectins, Arabic gum, commercial gums and extracts from various algae, corncob xylans, the hydrolysates and large and small molecular fractions of the above samples, Graptopetalum paraguayens extracts, and methylated derivatives of red algal polysaccharides. The results of antioxidation showed that the antioxidant activities such as DPPH-radical, ABTS-radical and superoxide-anions scavenging abilities increased with the concentration (110 mg/mL) for most of original polysaccharides. Among the samples examined, corncob xylans (Xyl B), G. paraguayens polysaccharide (GE) and hot-water extract (GW) showed the greatest ability in scavenging ABTS radicals and superoxide anions (both reach 100%, where GE exhibited the greatest ability in chelating DPPH radicals (66.9%). For the fractions of polysaccharide hydrolysates, the large molecular fractions of xylan hydrolysates (Xyl >10 k) displayed the best effect on removing ABTS radicals (up to 100%) and superoxide anions (>95%). In the case of Caco 2 cell inhibition experiments, the results at 1 mg/mL concentration indicated that Ulva arasakii polysaccharides revealed the greatest inhibition efficiency (89.7%), followed by GE (83.9%), the large molecular fraction of wheat bran glucans (BG>10 k) (80.7%), large molecular fraction of -carrageenan (B>10k) (74.6%), low molecular fraction of high-methoxy pectin hydrolysates by enzyme (HMP1-10k) (67.6%). For the derivatives of red algal polysaccharides, the inhibition effectiveness of -carrageenan acid hydrolysates (A20) (59.1%) was higher than the others. In conclusion, corncob xylans, wheat bran glucans, and GE showed excellent effects in both antioxidation and Caco-2 cell inhibition. Ulva polysaccharide exhibited the best Caco-2 cell inhibition activity. It is worthy to further investigate the bioactivity mechanisms of these samples. The other polysaccharide gums and extracts with various degrees of physiological activities are also important for developing novel healthy foods.
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