Proteomic analysis of cabbage under heat and flooding stresses

• Main Authors: Yuan Hao Lo, 羅元浩
• Other Authors: Hsiao Feng Lo
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/71069769792375410198

碩士 === 中國文化大學 === 生物科技研究所 === 96 === Cabbage (Brassica oleracea L. var. capitata L.) is one of the world important vegetable crops and Taiwanese popular vegetables. High temperature and rains in summer make cabbage under heat and flooding stress, and influence the yield and quality of summer production seriously. Improving heat and flooding tolerance of cabbage is one of the research targets. Heat- and flooding-tolerant ’Sha-sha-jieu’ and sensitive ’Mi-ni’ of cabbage were used as experimental materials. Forty-five days plants were treated separately by 22℃and 40℃with flooding and non-flooding stress in a phytotron for 0, 6, 12, 24, 48, 72 hours. In each timing of each treatment, growth characters, fluorescence parameters and stomatal conductance were measured. Protein profiles of leaves were resolved by ProteomeLabTM PF-2D (Beckman Coulter) and identified by Matrix assisted laser desorption/ionization- time of flight mass spectrometer (MALDI-TOF MS). On stressed leaves of ‘Mi-ni’, change of top fresh weight and dry weight, stomatal conductance, symptoms and Fv/Fm decrease were more serious than ’Sha-sha-jieu’. According to the above result, the leaves of 6, 12 and 24 hours treatment were chosen for PF-2D analysis. Through MALDI-TOF MS, 5, 9 and 20 different peaks in ‘Mi-ni’ stressed leaves treated for 6, 12 and 24 hours were respectively identified. The identified identical proteins included homocysteine methyltransferase, Sulfite reductase [NADPH] hemoprotein beta-component, Isoleucyl-tRNA synthetase, ATP synthase subunit beta, ribulose 1,5-bisphosphate carboxylase/oxygenase large chain, Ribulose bisphosphate carboxylase large chain precursor, rubisco ssu precursor, Oxygen-evolving enhancer protein 1-1, Oxygen-evolving enhancer protein 1-2, ATP synthase subunit beta, periplasmic binding protein/ LacI transcriptional regulator, Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes, L-lactate dehydrogenase and glycoside hydrolase, family 3 domain protein. In ‘Sha-sha-jieu’ stressed leaves treated for 6, 12 and 24 hours, 5, 9 and 20 different peaks were respectively identified. The identified identical proteins included Ribulose bisphosphate carboxylase large chain precursor, Cysteine desulfurase, ATP synthase subunit beta, Probable fucosyltransferase 8, putative hydratase/decarboxylase, V-type ATP synthase alpha chain, Oxygen-evolving enhancer protein 1-1, Oxygen-evolving enhancer protein 1-2, Heat shock 70 kDa protein, Serine/threonine protein phosphatase 7 inactive homolog, Cysteinyl- tRNA synthetase, Galectin-2 and Flagellin A. These proteins are related to basic metabolic pathways such as ATP production, carbohydrate metabolism, gene regulation, photosynthesis, protein biosynthesis, oxidative stress tolerance.