The evaluation of chitosan- CPTMS hybrid and its caffeic acid grafting derivatives used for bone tissue engineering scaffold

• Main Authors: Jia-Ching Shiu, 許佳慶
• Other Authors: none
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/39995535403067860266

碩士 === 國立聯合大學 === 化學工程學系碩士班 === 96 === Using chitosan (CS) as base material, sodium chloride particles as porogen, and (3-Chloropropyl)trimethoxysilane (CPTMS) as crosslinking agent, a novel CSC porous hybrid was prepared. Caffeic acid was grafted onto CSC to have a graft copolymer. The assessment of the CSC and CCA applied as bone tissue engineering scaffold was evaluated. SEM analysis indicated CSC has porous morphology with interconnected pores and pore size ranging in 80μm to 250μm. FTIR spectra showed the covalent linkage of CS with CPTMS and CSC with caffeic acid. DSC curve appeared CSC with higher water affinity than CCA . The CCA exhibited better antibacterial and antioxidant property than CSC. The compressive strength of CS was improved by crosslinking with CPTMS and further improved by copolymerizing with caffeic acid. The compressive strength of all the scaffolds was promoted after mineralized by calcium phosphate. The scaffolds were biodegradable in lysozyme solution. The degrading rate by lysozyme was in the order of CS > CSC > CCA. An osteoblast-like cell, rat osteogenic sarcoma, was cultured with CSC and CCA. The attachment of the cells with the scaffolds was observed by SEM photographs. After 3 days and 7 days of culture the cells attachment to CSC was observed better than on CCA. The hydrophobicity decreased the cells compatibility of CCA . After mineralization of CCA in simulated body fluid (the product was denoted as CCA-D), the hydrophilicity and the cells attachment of CCA were enhanced. This study shows CSC and CCA-D are promising candidates for bone tissue engineering scaffolds.