Studies of novel hydrophilic-modified nonwoven on antimicrobial control

• Main Authors: Chih-Sheng Yang, 楊智升
• Other Authors: Shuen-Fuh Lin
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/vdquf9

碩士 === 國立高雄大學 === 生物科技研究所 === 96 === We have discovered a kind of mold “RP-46” in our laboratory; it could secrete extracellular pigment. The main expression color of this pigment is red. Based on this research that red-antibiotics could suppress Gram-positive bacteria specifically, we commissioned Kaohsiung Veteran General Hospital to apply the experiment. The red-antibiotics could restrain several severe infections (such as ORSA, VRE, MDR and Mycobacterium tuberculosis, etc.) in hospital successfully when it was in extremely low density. We have refined the red-antibiotics of RP-46; This structure has been also analyzed, one of the compounds is quinolone’s structure which owns the functional base of the double carbonyl. It might be a marvelous anti-tuberculosis medicine in incoming future. We had been able to coat the red-antibacterial covalence on the nonwoven, the most suitable manipulative conditions is down below: Processing it for 60 minutes by 25% glutardialdehyde, 90°C and 150 rpm is the first step; and then after washing by acetone, processing it for two hour again by 20% PEI, 40°C and 150 rpm is the second step. The last step of processing schedule to get the best stable performance is adding the red antibacterial substance and shaking it for one day by 150 rpm 30°C constantly. After dealing with glutardialdehyde and PEI, and the continuous processing steps for 6 times (Each time it needs to be soaked for 12 hours approximately), the nonwoven could still keep its antibacterial activeness. It was obvious to see that the method of glutardialdehyde and PEI copolymerization could be able to firm the red-antibiotics on the nonwoven efficiently. We had found that the nonwoven with stationary red-antibiotics and the targeting bacteria stuck together throughout the SEM examination on the bacteriostasis’ test. It showed its function was related with cell wall binding.Because there is still a non-responded carbonyl on the nonwoven, therefore it could connect with enzyme and form a particular construction like a sandwich. It might be applied to the biosensor and the artificial dressing in the future.