Analysis and Control of the Fed-batch Culture Process of Recombinant Escherichia coli

• Main Authors: JHANG, HUEI-YU, 張卉瑜
• Other Authors: Yi-Shyong Chou
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/13360786443212008707

碩士 === 國立臺灣科技大學 === 化學工程系 === 96 === Recombinant E. coli is induced under the control of the promoter, by feeding the lactose used as the inducer which can induce and express recombinant protein. A model-based feed-rate profile optimization problem is discussed for the fed-batch recombinant protein production. The optimization procedure, applying Genetic Algorithm, one of Evolutionary Algorithm methods, is discussed the results of the optimization that contains the two objective functions regarded as the fewest amount of the lactose feed and the highest amount of recombinant protein. After the optimization, we use the optimal lactose feed-rate to design Single-input Single-output（SISO）control. In the actual process with disturbance, the control is perfectly performed both by PI controller and MPC.