| Summary: | 碩士 === 國立臺灣科技大學 === 化學工程系 === 96 === In this research, the adjustable LED (Light Emitting Diode) light with various energy and wavelength was used as light source to stimulate osteoblast-like cells, UMR-106. The responses of osteoblastic cells were investigated, including MTT assay, total protein synthesis, cell proliferation and cell attachment. Also, the ALPase (Alkaline phosphatase) activity and calcium deposition were also tested for the relazation of osteoblastic differentiation.
The wavelengths used in this study included 652 (red light), 590 (yellow light) and 415 nm (blue light) and the powers were 2.71, 3.89 and 4.17 mW/cm2, respectively. The energy for LED stimulus was ranged from 0.1 to 20 J/cm2. The experimental outcomes indicated that the optimal stimulation energy of blue light (0.5J/cm2) can promote the mitochondria activity, cell proliferation and expression of osteoblastic markers. However, the effective energy of red and yellow lights was higher (5J/cm2). The possible reason would be that photo-acceptors for lights with different wave length are different, so the required energy levels also diverse. On the other hand, to the light stimuli with high energy (10~20 J/cm2) would inhibit the cell activity and differentiation, no matter which wave length was used. The importance of stimulation power and energy was also investigated in this study. The results suggested that the irradiation energy is dominant, compared with the stimulation power.
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