Synthesis of amphipathic polymer by using cellobiose for gene delivery

• Main Authors: Hau-yu Lin, 林皓譽
• Other Authors: Wen-Chi Tseng
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/73682410004663218743

碩士 === 國立臺灣科技大學 === 化學工程系 === 96 === In non-viral vector system, avoided safety and immunity viral vector of viral vector which used liposomes and cationic polymer to be a vector of gene. Hydrophilic highly, stable and biocompatible hightly cellobiose and hydrophobic and biocompatible hightly 12-aminododecanoic acid were formed an amphipathic polymer. Amphipathic polymer were used as major DNA carrier. At first, there were three materials for synthesis formed an amphipathic polymer .We used DNA of 0.5 μg/μl to do DNA electrophoresis, and investigated how the ability of the carrier bound DNA. And then, we researched the different N/P ratio of materials, and compared the ability of binding DNA with each other. As to three kinds of amphipathic polymer, it was obvious that carrier bound DNA for CA-ADA-A-BA. But it could not bind DNA completely. However, it was interesting in ADA-BA.ADA-BA could be bound by DNA. In this study, we used CA-ADA acid to be formed amphipathic polymer. And then, it could not bind DNA completely. After removing this molecule of cellobiose, it had batter binding effective than CA-ADA-A-BA. In future, we should design higher electric charge of hydrophilic and hydrophilic molecule or longer carbonic chain to achieve optimal design.