| Summary: | 碩士 === 國立臺灣大學 === 生物產業機電工程學研究所 === 96 === In this study, swine manure was converted by Pleurotus ostreatus and Lactobacillus delbrueckii subsp. bulgaricus through the two-stage fermentation. In the first stage, the dry swine manure was inoculated with Pleurotus ostreatus and were fermented at 30℃ during 1~6 weeks. The products of the first stage were then dried and autoclaved to be the substrates of the second stage. In the second stage, 5% glucose was added as the feed supplement besides the products of the previous stage and were cultured with L. delbrueckii subsp. bulgaricus at 37℃ for 3 weeks. Also, another set of experiments without glucose addition were tested as the control experiments.
In the first stage, the crude fat content were decreased during the period of fermentation were observed the highest reduced sugar concentration (2.78 g/L) at the third week and the crude protein content of the fifth week was 9.61% (w/w) which showed a 1.84% increase from the beginning.
In the second stage, it seemed that the products of three weeks fungi fermentation could generate enough feed for Lactobacillus. After the second stage fermentation, the crude protein increased 0.5% and the bacteria count of Lactobacillus could maintain at a level of 106 CFU/mL without glucose addition. Although the crude protein content and the bacteria count were also good for those glucose addition experiments, it was not economical for extra change of the chemicals.
In conclusion, the pretreatment of fungi could beneficially convert the swine manure to some short chain materials and hence increased the digestion efficiency of the consequent Lactobacillus fermentation.
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