| Summary: | 碩士 === 國立臺灣大學 === 生物產業機電工程學研究所 === 96 === Polymerase Chain Reaction (PCR) was issued so far in 1986; it made genetic engineering technology fast growing. Because Micro-Electro-Mechanical System (MEMS) grows vigorously in recent years, it has made Polymerase Chain Reaction change significantly in reducing size scale, lowering energy consumption, and speeding up the process.
The process of Polymerase Chain Reaction needed fixed and stable temperature. In order to make heat transfer faster, using the silicon wafer as the material of PCR chip can do the following: utilizing photolithography process, etching process and anodic bonding process in MEMS process, to reduce the size of PCR chip. This is the main purpose of this research. This research also utilizes simulation to analyze the heat distribution of the chip, and to solve the interference question of temperature between three different temperature zones of the PCR chip.
The research begins with using MEMS technology and PDMS process attempting to make the first micro-flow chip. The results had shown it could simplify the temperature controlling device, but sometimes the DNA reactant would seep from the joint of silicon and PDMS. Thus the heaters and temperature sensors were plated with Pt and Cr to the second edition micro-flow chip by MEMS technology and packaged the pyrex 7740 glass and silicon wafer by anodic bonding process to solve the problem of reactant seepage. Finally, simulation software has found the optimal width and depth of the isolation zone.
From the testing results, DNA reactant in PCR Chip can really be heated to appropriate temperature, and be in process of Polymerase Chain Reaction. Because the injection hole at the PCR chip was unable to connect closely with external syringe, it causes reactant waste when inject into the PCR chip. Research should continue on the encapsulation of PCR chip in order to solve the problem mentioned above.
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