| Summary: | 碩士 === 國立臺灣大學 === 微生物與生化學研究所 === 96 === In this thesis, we try to develop a new surface plasmon resonance (SPR) immunobiosensor to detect Influenza A/ H1N1. We combine di-para-xylene (Parylene) and the SPR system to enhance the system stability and sensitivity. Parylene has the property of surface regularity, low permittivity, electric stability, and biocompatible. It is hydrophobic and it can resist the damage caused by acid and bases. Those characters make it a good physical, chemical, and biological barrier material. Because Parylene is very stable material, we use hydrophobic plasma to modify the surface of Parylene chip. Silano compound has a positive electricity group end and can form a self-assembled monolayer (SAM) on the chip. The nanogold particals were attracted to the silano surface by the static electricity. Then we used different linkers to immobilize the antibody to make the SPR chip. The linkers are 3-MPA (3-mercaptopropionic acid), 11-MUA (11-Mercaptoundecanoic acid) and16-MHA (16-Mercaptohexadecanoic acid). They have different number of carbon but have similar structure that one end is thiol group which can form S-Au bond, and the other end is carboxyl group. The immobilization of antibodys were carried gut by activation of the surface carboxyl groups with NHS (N-Hydroxysuccinimide) and EDC (N-Ethyl-N''-(3-dimethylamino-
propyl)carbodiimide polymer) to form the NHS ester and by displacement of the NHS ester with an amino group on the antibody to form an amide bond. Furthermore, we used mask to make a grating on the chip to improve the reactivity. The SPR system offer many advantages including cheap, resisting electric interference, stable, and high reactivity. According to our study, the system has the potential to operate in complex condition, and it could apply other model biosensor for epidemic control and clinical detection.
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