| Summary: | 碩士 === 國立臺灣大學 === 工程科學及海洋工程學研究所 === 96 === As binding and even conformation changes often appear in bio-molecule interactions, adding more measurement parameters can facilitate the analysis of such a complex reaction process. Quartz Crystal Microbalance(QCM) provides us with measurement capabilities on two such important parameters, i.e., frequency change and dissipation factor. In this study, Surface Plasma Resonance(SPR) was added to QCM to create a measuring system with the third parameter of interest. That is, optical power change from SPR can be simultaneously measured with frequency change and damping factor. In addition to observe the reaction process of bio-linker and gold film through this new construction. The difference between the QCM and SPR were compared as well.
QCM and SPR are both well-developed biosensors. They both have high sensitivity even though they were developed with different measurement principle. With each of the two methods possess their own advantageous research fields, their pros and cons were compared from time to time. The most common approach is to compare each of these two methods independently. However, this kind of comparison suffers from the influence of different environmental conditions. A better approach is to perform the comparisons in-situ, simultaneously, and side by side. It is to be noted that since SPR is typically activated by method of grating coupling, which demands lithorgraphic process on to the gold film deposited on the quartz. In other words, it will make the comparison time consuming and costly.
In this thesis, we developed a direct and easy to operate method to perform the above-mentioned comparisons. More specifically, we have integrated both QCM with SPR into a single device in this study. In our design, QCM is placed directly on the prism through coupling oil and SPR is activated by prism coupling. The electrode of quartz which contacts the prism is replaced with ITO for light beam transmissions. The simplicity provides us with a new instrument that maintains the benefit of both instruments, which simultanesously enables the measurement of integrated parameters for sensing and a platform for functional comparisions.
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