Expression of Clock Genes in Cockroaches

博士 === 國立臺灣大學 === 昆蟲學研究所 === 96 === The distributions of immunoreactivity (ir) cells against three clock-related proteins (Period, PER; Pigment Dispersing Factor, PDF; and Corazonin, CRZ) were compared between two species of cockroaches. The German cockroach, Blattella germanica, and the double-str...

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Main Authors: Chih-Jen Wen, 溫志仁
Other Authors: How-Jing Lee
Format: Others
Language:en_US
Published: 2008
Online Access:http://ndltd.ncl.edu.tw/handle/13568332129197728449
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spelling ndltd-TW-096NTU051850132015-11-25T04:04:25Z http://ndltd.ncl.edu.tw/handle/13568332129197728449 Expression of Clock Genes in Cockroaches 時鐘基因在蜚蠊之表現 Chih-Jen Wen 溫志仁 博士 國立臺灣大學 昆蟲學研究所 96 The distributions of immunoreactivity (ir) cells against three clock-related proteins (Period, PER; Pigment Dispersing Factor, PDF; and Corazonin, CRZ) were compared between two species of cockroaches. The German cockroach, Blattella germanica, and the double-striped cockroach, B. bisignata, are sibling species with similar period sequence but distinctive circadian rhythm in locomotion. Three major groups of PER-ir cells are located in optic lobes (OLs), which are the sites of the major circadian clock. They are interconnected with PER-ir neural bundles. Interestingly, the potential output signal of the circadian clock, PDF, is co-localized with PER in all three groups of cells. However, only two CRZ-ir cells and their processes are found in the OLs and they are not co-localized with PER-ir or PDF-ir cells and processes. Since only one circadian rhythm is expressed in locomotion, the time signals from both major clocks in the OLs are coupled by connection with PDF-ir processes. A group of 3-4 PER-ir cells in the protocerebrum display typical characteristics of neurosecretary cells. In addition, there are numerous, small PER-ir and PDF-ir co-localized cells in the pars intercerebralis (PI) which have direct connections with the neurohemoorgan, corpora cardiaca (CC), through PER-ir and PDF-ir processes. Based on these findings, the cellular connection shows a circadian control through endocrine route. For the rest of central nervous system, only a few PER-ir and PDF-ir cells or processes are detected. This finding implies the circadian clock for locomotion is not located in subesophageal ganglion (SOG), thoracic or abdominal ganglia, but may use other neural messengers to pass on circadian signals. Since the overall distribution pattern of the clock cells are the same for B. germanica and B. bisignata, the possible explaination of the different expression of locomotion between the species depends on genes downstream of per, pdf and crz. The appendix at the end of the thesis is the the article “Distribution of Corazonin and Pigment-Dispersing Factor in the cephalic ganglia of termites”, which is accept by the journal “Arthropod structure and development”. We finished this work about termites by teamwork with our coauthors: Dr. Ivo Sauman, Dr. František Sehnal, Dr. Ivan Hrdý and Dr. Radka Závodská of Czech Republic. How-Jing Lee 李後晶 2008 學位論文 ; thesis 96 en_US
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description 博士 === 國立臺灣大學 === 昆蟲學研究所 === 96 === The distributions of immunoreactivity (ir) cells against three clock-related proteins (Period, PER; Pigment Dispersing Factor, PDF; and Corazonin, CRZ) were compared between two species of cockroaches. The German cockroach, Blattella germanica, and the double-striped cockroach, B. bisignata, are sibling species with similar period sequence but distinctive circadian rhythm in locomotion. Three major groups of PER-ir cells are located in optic lobes (OLs), which are the sites of the major circadian clock. They are interconnected with PER-ir neural bundles. Interestingly, the potential output signal of the circadian clock, PDF, is co-localized with PER in all three groups of cells. However, only two CRZ-ir cells and their processes are found in the OLs and they are not co-localized with PER-ir or PDF-ir cells and processes. Since only one circadian rhythm is expressed in locomotion, the time signals from both major clocks in the OLs are coupled by connection with PDF-ir processes. A group of 3-4 PER-ir cells in the protocerebrum display typical characteristics of neurosecretary cells. In addition, there are numerous, small PER-ir and PDF-ir co-localized cells in the pars intercerebralis (PI) which have direct connections with the neurohemoorgan, corpora cardiaca (CC), through PER-ir and PDF-ir processes. Based on these findings, the cellular connection shows a circadian control through endocrine route. For the rest of central nervous system, only a few PER-ir and PDF-ir cells or processes are detected. This finding implies the circadian clock for locomotion is not located in subesophageal ganglion (SOG), thoracic or abdominal ganglia, but may use other neural messengers to pass on circadian signals. Since the overall distribution pattern of the clock cells are the same for B. germanica and B. bisignata, the possible explaination of the different expression of locomotion between the species depends on genes downstream of per, pdf and crz. The appendix at the end of the thesis is the the article “Distribution of Corazonin and Pigment-Dispersing Factor in the cephalic ganglia of termites”, which is accept by the journal “Arthropod structure and development”. We finished this work about termites by teamwork with our coauthors: Dr. Ivo Sauman, Dr. František Sehnal, Dr. Ivan Hrdý and Dr. Radka Závodská of Czech Republic.
author2 How-Jing Lee
author_facet How-Jing Lee
Chih-Jen Wen
溫志仁
author Chih-Jen Wen
溫志仁
spellingShingle Chih-Jen Wen
溫志仁
Expression of Clock Genes in Cockroaches
author_sort Chih-Jen Wen
title Expression of Clock Genes in Cockroaches
title_short Expression of Clock Genes in Cockroaches
title_full Expression of Clock Genes in Cockroaches
title_fullStr Expression of Clock Genes in Cockroaches
title_full_unstemmed Expression of Clock Genes in Cockroaches
title_sort expression of clock genes in cockroaches
publishDate 2008
url http://ndltd.ncl.edu.tw/handle/13568332129197728449
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