| Summary: | 碩士 === 國立臺灣大學 === 化學工程學研究所 === 96 === Granules can be described as a collection of sel-immobilized cells and extracellular polymeric substances (EPS) into a somewhat spherical form. EPS can mediate both cohesion and adhesion of cells and play a crucial role in maintaining the structural integrity in a community of immobilized cell and the composition of EPS include proteins, polysaccharides and lipid .In recently research, the presence of divalent and trivalent cations ions, such as Ca2 + etc, helps cells to form microbial nuclei that promote further granulation
The investigation into structural stability of three biochemical granules (one aerobic granules and two anaerobic granules) were examined. Selective enzymes of proteins, lipids, proteinase K, α- amylase, β- amylase, cellulase and EDTA were added to those three granules to influence structural stability. The roles of proteins,α- and β-polysaccharides, and lipids were studied via their selective hydrolysis using enzymes and structural changes of granule were probed using in situ fluorescent staining and confocal laser scanning microscopy.
The results show elective enzymes of proteins, lipids, proteinase K, α amylase, β amylase and EDTA are not backbone of those three granules.Also, cellulose are not backbone of methane production by anaerobic granules and Degradation of Phenol by Aerobic Granules. However, cellulose form the backbone of a network-like outer layer with embedded proteins, lipids, a-polysaccharides, and cells to support the mechanicalstability of structural integrity of hydrogen production by anaerobic granules.
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