In vitro antioxidant properties, human skin fibroblast UVA protection of crude rosmarinic acid and its applications in cosmetics

• Main Authors: Yi-Chieh Chu, 朱怡潔
• Other Authors: Rong-Huei Chen
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/38725444205300702036

碩士 === 國立臺灣海洋大學 === 食品科學系 === 96 === The objective of the study are to explore the efficacy of an anti- photoaging skincare that containing the crude rosmarinic acid of Origanum vulgare L.. The study includes 1. To evaluate the efficacy of antioxidation of crude rosmarinic acid by observation the scavenging capacity of DPPH radical, reducing power, scavenging effects on hydrogen peroxide, 2. Determining cells viability, photoprotection and restoration, collagen formation of the cultured fibroblast. 3. To prepare and characterize the cream containing 0.5% crude rosmarinic acid. The characteristics of cream studied including storage stability, the safety of the products, water holding capacity, the skin elasticity, skin roughness, skin scaliness, skin smoothnes, and skin wrinkles index. Scavenging capacity of DPPH radical, reducing power, scavenging effects on hydrogen peroxide increased with increasing the concentration of crude rosmarinic acid. The 1% crude rosmarinic acid had the highest (87.49±1.46%) DPPH scavenging effect. The concentration of crude rosmarinic acid greater than 0.25% showed more than 80 % scavenging effects on hydrogen peroxide. Crude rosmarinic acid concentration ranged between 0.5-1% showed the highest reducing power. Cell viability results showed crude rosmarinic acid concentration rangad between 3.13-100 μg/ml has no effect on CCD-966SK viability. The optimum concentration of crude rosmarinic acid that showed protect and restore capacities on photo-damaging by UVA was 25-50 μg/ml promoted the cell proliferation significant. Crude rosmarinic acid concentration ranged between 6.25-50 μg/ml enhanced higher collagen formation of CCD-966SK than that of the control and collagen increased with increasing crude rosmarinic acid concentration after growth for 24 hr. Cream product containing without crude rosmarinic acid was higher in whiteness than that containing 0.5% crude rosmarinic acid. The stabilities of both cream were stable for more than four months storage, at various temperature environments; after sonication, or centrifuging treatment. The pH of cream products did not change significantly. These results showed that the stability of cream products were very good. The safety test resulted in no erythema based on the Daize score test. The skin appearance such as water holding capacity, the skin elasticity (R2), and the skin smoothnes index significantly increased for applying sample containing 0.5% crude rosmarinic acid during six weeks test period. The skin roughness, skin scaliness and skin wrinkles index significantly decreased over time during six weeks test period.