Preparation and Biofunctional Properties of Water-Soluble Chitosan-Laminaria japonica Crude Polysaccharide Nanoparticles and its Application in Cosmetics

• Main Authors: Yo-Ru Hsu, 許攸如
• Other Authors: Rong-Huei Chen
• Format: Others
• Language: en_US
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/46630483593934464983

碩士 === 國立臺灣海洋大學 === 食品科學系 === 96 === Laminaria sp. is the largest throughput with important economical among cultivated macroalgaes in the world, and Taiwan is the main import country. The aims of this study were to prepare a new nanoparticle (Laminariasphere) system via ionic gelation of Laminaria japonica crude polysaccharide hydrolysate and low molecular weight chitosan (LMCS), and to evaluate effect of LJCPH and LMCS-LJCPH nanoparticles on cell viability and collagen secretion capability on cultured human normal skin fibroblast (CCD-966SK) and to finding out the optimal concentration for skincare products. An anti-aging skincare containing the LMCS-LJCPH nanoparticles was prepared and its characteristics including storage stability, safety and effecacy such as the skin elasticity, skin hydration, skin roughness and skin sensitive were measured. The results obtained were that both crude polysaccharide (LJCP) and crude polysaccharide hydrolysate (LJCPH) from Laminaria japonica showed anti-oxidation capabilities. The functional compositions result shows LJCP were lower in total sugar, reducing sugar, polyphenol and flavonid than that of LJCPH, but has a similar sulfate content. Anti-oxidation activities data showed that anti-oxidation capabilies of LJCPH were better than that of LJCP and anti-oxidation capability in general increased by increasing the concentration of the sample. Low molecular weight chitosan (LMCS) were prepared by hydrogen peroxide (H2O2) degradation. The LMCS solution were prepared by varying the volume ratios, stirring time, stirring speeds to prepare nanoparticle of mean diameter of 100-200 nm, zeta potential at＋28 ± 3 (mV) and spherical shape observed by SEM. The ionic interations occurred between LMCS and LJCPH as elucidated by FT-IR spectra. There are no obvious change during storage at pH 5.5-6.6 solutions and 4, 25�aC for 28 day, the results shows that the nanoparticles is stable during storage period. LJCPH and LMCS-LJCPH nanoparticles at 62.5-10000 μg/ml have higher cell viability than control. The collagen secretion capability of the cultured human normal skin fibroblast increased when the concentration of LJCPH and LMCS-LJCPH nanoparticles at 250 μg/ml. The enhancement were more pronounced of LMCS-LJCPH nanoparticles on collagen secretion capability than that of LJCPH. Proliferation effect of LMCS-LJCPH nanoparticles on Keratinocyte (HaCaT) cell viability is higher than that LJCPH. pronoumced The cosmetic products containing either with or without LMCS-LJCPH nanoparticles were stable after sonication (135 W, 42KHz, 30 min), after centrifugation (3000 rpm, 30 min), and after more than four storage months at high temperature (50 �aC). The safety test resulted in no erythema based on the Daize score test. Skin eleasticity (R2) for those whom have applied the cosmetic product containing LMCS-LJCPH nanoparticles increased over time during six weeks test period. Skin roughness decreased over time during six weeks test period and did show a significant difference for skin scaliness, skin wrinkles index, skin smoothnes and skin roughness.