Population genetic study and Classification of Polypedates leucomystax species complex in Taiwan area

• Main Authors: Tian-You Zhang, 張天祐
• Other Authors: Kuang-Yang Lue
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/sdzars

碩士 === 國立臺灣師範大學 === 生命科學研究所 === 96 === Hong Kong Whipping Frog(Polypedates megacephalus) widely distributes in the East Asian area. Because to differentiate condition variation is difficult, there are many disputes as well as the scotoma in its classified status. The partial sequence of mtDNA cytochrome b (Cyt-b)& nDNA Rhodopsin was used as genetic marker in this study. The length of sequenced Cyt-b gene was 615bp and Rhodopsin gene was 358bp. Totally, 191 specimens were obtained in Taiwan. This research using the Cyt-b sequence to analyze and discuss genetic structure of Polypedates megacephalus in the island of Taiwan(besides the Zhanghua Tianwei area). There are two haplotype(Taiwan I & Taiwan II) groups in Taiwan according as minimum spanning network. The variation of each haplotype is very small. It is the most possible that there are two mainland ancestral populations invade to Taiwan island. Phylogenetic trees generated from the ML、NJ and MP methods showed that there are another specie frogs (P. sp.) in Taiwan & Guizhou. The populations of Hainan Island, Mazu, Hong Kong & Zhanghua Tianwei area are real P. megacephalus. We discovered the proportion size of head and the calling have the obvious difference between P. sp.& P.megacephalus.