| Summary: | 碩士 === 國立臺灣師範大學 === 生命科學研究所 === 96 === Unilateral obstructive uropathy refers to the structural changes, like ureteral cot, stenosis, tumor and damage, that impede the normal flow of urine and the most common cause is renal calculus. Unilateral ureteral obstruction (UUO) is an animal model of mimic progressive fibrosis renal disease that can reproducibly demonstrate this pathological progress. The aim of the present study was to investigate the role of reactive oxygen species , apoptosis, autophagy and the relation between apoptosis and autophagy in UUO model. In this research, female wistar rats were performed ligation of the right ureter at the ureteropelvic junction using double silk suture. Rats were divided into 8 groups and the first group had no UUO operation and others were sacrificed after operating UUO for 4hrs, 8hrs, 12hrs, 1 day, 3 days, 5 days, 7 days. After then, rats were proceeded with physiological observation, 500μl volume of renal venous blood for free radical detection, harvested obstruction kidney for protein expression with western blot assay and immunohistochemistry stain for morphology changes. Our results found that, renal venous blood had increased hydrogen peroxide/hydroxyl radical production after UUO 4hrs to 12hrs. Catalase and MnSOD protein expression was decreased with a time-dependent manner from obstruction 4hrs. Bax and Bcl-2 ratio and Caspase 3 protein expression of UUO kidney was increased. UUO enhanced autophagy step Ⅰ protein, Beclin-1； step Ⅱ protein, Atg5-Atg12 complex； step Ⅲ protein, LC3 Ⅱ in a time-dependent after 3 days of UUO. We observed an incremental autophagy and apoptosis protein expression in renal proximal and distal tubules by immunohistochemistry. These results suggest that autophagy and apoptosis have identical tendency and represents that autophagy may play a role with apoptotic cell death in UUO animal models.
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