Fabrication of the silicon nanowire field-effect transistors:detection of glutathione S-transferases (GSTs) and the washing efficiency of washing bufferby SiNW FETs

• Main Authors: Shu-Han Yu, 于書翰
• Other Authors: 孫英傑
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/65990867360798666242

碩士 === 國立臺灣師範大學 === 化學系 === 96 === Small GTP binding proteins, also called G-proteins, for example Rab3A, are involved in modulating many physiological activities, including gene expression, cytoskeleton rearrangement, exocytosis, etc. These G-proteins have GTPase activity and normally are inactive bound with GDP; when activated, GDP will be exchanged with GTP to allow the G-proteins to interact with downstream proteins. Exocytosis, endocytosis and transcytosis are performed by intracellular vesicle trafficking. Exocytosis secretion of neurotransmitters and hormones is a fundamental step in synaptic neurotransmission and cell-cell communication and involves sequential steps of complex protein-protein interactions. In this experiment, we used GSH for the surface modification of silicon nanowires. And we then conducted a real-time, label-free electrical signal measurement for GST biosensing, and test of the washing efficiency with washing buffer by silicon nanowire field-effect transistors. Detection and quantification of biological and chemical species are central to many areas of healthcare and life sciences, ranging from diagnosing disease to the discovery and screening of new drug molecules. Semiconductor nanowires configured as electronic devices have emerged as a general platform for ultra-sensitive direct electrical detection of biological and chemical species. Here we describe a detailed protocol to fabricate nanowire electronic sensors. First, the growth of uniform, single crystal silicon nanowires, and subsequent isolation of the nanowires as stable suspensions are outlined. Second, fabrication of addressable nanowire device arrays is described. Third, modification of the nanowire device surfaces with receptors is described. Fourth, an example modification and measurements of the electrical response from devices are detailed. The silicon nanowire (SiNW) devices have demonstrated applications for label-free, ultrasensitive and highly-selective real-time detection of a wide range of biological and chemical species, including proteins, nucleic acids, small molecules and viruses.