Micro Array Stamping System for Passive Batch-filling and In-Parallel-Printing Protein Solutions

• Main Authors: Ho, Cheng-En, 何承恩
• Other Authors: Tseng, Fan-Gang
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/44390434863122623149

博士 === 國立清華大學 === 工程與系統科學系 === 96 === This work develops a novel micro contact printing system for printing tens of protein solutions into an array with batch filling and parallel printing. This printing system consists of a micro filling chip and a micro stamp chip. The micro filling chip can simultaneously transfer numerous protein solutions into the micro stamp chip in seconds by capillary force without cross-contamination, while preserving the functionality of proteins. Different proteins can be dispensed into the corresponding channels and driven into the tips of the micro stamps. The micro stamp can be then brought to contact with the substrate to produce bio-fluid spot arrays. Teflon patterns are applied on both the micro filling chip and the micro stamp chip to prevent cross-contamination during filling. Thirty-six proteins can be printed in parallel with a spot size variation of less than 5%. This device has a potential to be expanded to a passive and high throughput system for simultaneously printing hundreds of bio-fluid spots to form dense arrays for diagnosing disease or screening for drugs.