Image Stitching of the Drosophila Brain Images from Confocal Microscope

碩士 === 國立清華大學 === 電機工程學系 === 96 === By research on fruit flies’ brain circuits in life science, we could possibly get more information about how human brain works. One approach is to use high-resolution microscopy to take pictures of fruit flies’ brain slices of two or more images with overlap to ea...

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Bibliographic Details
Main Authors: Yao-Hsuan Tsai, 蔡耀萱
Other Authors: Yung-Chang Chen
Format: Others
Language:en_US
Published: 2008
Online Access:http://ndltd.ncl.edu.tw/handle/62796699940089328117
Description
Summary:碩士 === 國立清華大學 === 電機工程學系 === 96 === By research on fruit flies’ brain circuits in life science, we could possibly get more information about how human brain works. One approach is to use high-resolution microscopy to take pictures of fruit flies’ brain slices of two or more images with overlap to each other. Combining these images is the first step to start the research on fruit flies’ brain circuits. To reach the goal, we completed the work in two stages. In the first stage, the corresponding points in the two or more to-be-combined images are identified and registered. This part is composed of finding opposing positions of the images on x-y plane and taking adjacent images at z coordinate into consideration. In the second stage, the intensities of these images are blended. To eliminate the artifact of the seam as little as possible, distance map is computed. Besides, the intensity compensating method and the multi-band blending method are used. The experimental results show that the performance of the proposed method makes no requirement of setting many parameters and gives satisfactory results.