Determination of the triarylmethanes and corresponding metabolites in aquatic animal tissues by high-performance liquid chromatography-tandem mass spectrometry

• Main Authors: Ter-min Wang, 王德敏
• Other Authors: Wei-Hsien Wang
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/cqx6qm

碩士 === 國立中山大學 === 海洋生物科技暨資源學系研究所 === 96 === There are two purposes in this research, one is the development of the new method which can be used for detection and quantification of triarylmethanes in fish tissues. The other is that we confirmed validation and utility of triarylmethanes by the method that is according to Commission Decision 2002/657/EC. Homogenized fish tissues were extracted twice with acetonitrile and defatted with n-hexane. HPLC separation was conducted with the RP-18 column. The mobile phases consisted of 0.5 mM ammonium acetate buffer (pH 3.8, adjusted with acetic acid)– ACN (contained 0.1% formic acid) solution. Triarylmethane was determined by LC-ESI-MS/MS in positive mode. The correlation coefficients of calibration curves with triarylmethane in fish tissues were 0.998 ~ 0.999. The decision limits (CCα) were 0.16 ± 0.07 μg/kg(MG), 0.15 ± 0.04 μg/kg(LMG), 0.20 ± 0.13 μg/kg(CV) and 0.23 ± 0.12 μg/kg(LCV), and detection capabilities (CCβ) were 0.20 ± 0.09 μg/kg(MG), 0.18 ± 0.05 μg/kg(LMG), 0.24 ± 0.16 μgkg-1(CV) and 0.29 ± 0.15 μg/kg(LCV).