Investigation of pharmacokinetics of malachite green and leucomalachite green in Tilapia with liquid chromatography-tandem mass spectrometry

• Main Authors: Nai-yuan Lin, 林乃元
• Other Authors: Wei-Hsien Wang
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/vjh7w7

碩士 === 國立中山大學 === 海洋生物科技暨資源學系研究所 === 96 === The purpose of this research is that investigate the effects of time and concentration of exposure for the accumulation and depletion of malachite green and leucomalachite green in tilapia by pharmacokinetics. LC-MS/MS was used as the analytical instrument in this research, and the detection limit of malachite green and leucomalachite green is 0.51 ppb and 0.48 ppb. The results show that malachite green is unstable at high temperature. Addition of TMPD into the standard can stabilize malachite green. The malachite green in exposure water is easy to adhere to the fiberglass tub and cause the decreases of concentration of malachite green in water. The concentrations of malachite green and leucomalachite green in tilapia are positive related to the time and concentration of exposure. In experiments of exposure of malachite green, the highest concentration of malachite green occurs in liver, accumulation rate constant is 21.62 h-1. Liver is also the major organ for transforming malachite green into leucomalachite green, the net leucomalachite green accumulation rate constant is 213.67 h-1. In the period of water bath, Gill is the fastest organ for eliminating malachite green and leucomalachite green, the elimination rate constant is 0.7799 h-1 and 0.4658 h-1; the leucomalachite green concentration in fat is still increase until 6h of water bath.