Effects of Anti-tumor Drugs on OC2 Human Oral Cancer Cells

• Main Authors: Hsing-Hao Su, 蘇性豪
• Other Authors: Chung-Ren Jan
• Format: Others
• Language: en_US
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/6tqd2k

碩士 === 國立中山大學 === 生物科學系研究所 === 96 === The present study explored the effect of three anti-tumor drugs (cisplatin, fluorouracil, and temozolomide) on viability and cytosolic free Ca2+ concentrations ([Ca2+]i) in OC2 human oral cancer cells. The effect of cisplatin related mitogen-activated protein kinases (MAPKs) phosphorylation was also examined. Cisplatin at concentration of 25-150 μM decreased viability in a concentration-dependent manner, and so did fluorouracil (50-1000 μM) and temozolomide (50-600 μM). The three anti-tumor drugs all failed to induce a [Ca2+]i increase; thus it seemed that these drugs induced cell death via Ca2+-independent pathways. Immunoblotting showed that OC2 cells have background phospho-ERK, phospho-JNK and phospho-p38 MAPKs. It was found that cisplatin influenced the phosphorylation of ERK, JNK and p38 MAPKs at different time points.