Cloning and Expression of the Virulence Factors of Mannheimia (Pasteurella) haemolytica and Characterization of Their Antigenicities
碩士 === 國立屏東科技大學 === 獸醫學系所 === 96 === The bacterium Mannheimia (Pasteurella) haemolytica is a member of the Pasteurellaceae family. Pneumonic pasteurellosis is the major cause of death in fibrinous pneumonia. The disease can rapidly progress to fatality, thus prevention of disease by vaccination is t...
| Main Authors: | , |
|---|---|
| Other Authors: | |
| Format: | Others |
| Language: | zh-TW |
| Published: |
2010
|
| Online Access: | http://ndltd.ncl.edu.tw/handle/36329576700441100934 |
| Summary: | 碩士 === 國立屏東科技大學 === 獸醫學系所 === 96 === The bacterium Mannheimia (Pasteurella) haemolytica is a member of the Pasteurellaceae family. Pneumonic pasteurellosis is the major cause of death in fibrinous pneumonia. The disease can rapidly progress to fatality, thus prevention of disease by vaccination is the major strategy to control the disease. Several studies indicated that virulence factors of M. haemolytica are the most important antigens in stimulating resistance to pneumonic pasteurellosis. The purpose of this study is to clone and express the virulence factors of M. haemolytica and characterize their antigenicities. Our preliminary results reveal that the titers of the M. haemolytica specific antibody in serum were significantly increased at the 8th week after immunized with the inactivated pasteurellosis vaccine. Antibody were declined at the 12th week but they still could be detected until the 19th week. This result indicated that the traditional inactivated pasteurellosis vaccine can only provide a short term protection. Thus, we cloned the 38 kDa lipoprotein (Lpp38) and leukotoxin (Lkt) of M. haemolytica as potential immunogens. Their nucleotide sequences have been confirmed and the genes were expressed using the prokaryotic expression system. The antigenicity of Lpp38 fusion protein was evaluated the antibody titer, when Lpp38 fusion protein was used as potential immunogen to improve the inactivated pasteurellosis vaccine.
|
|---|