| Summary: | 碩士 === 國立屏東科技大學 === 獸醫學系所 === 96 === Salmonella is an important bacterium that causes diseases infecting the human alimentary tract, and also found in the excrement of the human, pets, poultry, domestic, and wild animals. It can be transmitted via the environmental pollution resulting in continuous exposure to the inhabitants. Increasing use of various drugs for the treatment of infectious diseases has resulted in an increase of drug-resistant clinical isolates. Recently, integrons and antibiotic-resistant gene cassettes are major factors in the development of multiple drug resistance among bacteria. Samples for this study were collected from August 2004 to December 2006 from a teaching hospital in Pingtung and about 215 bacterial strains were isolated. We perform a combination of the following tests : (1) polymerase chain reaction (PCR); (2) serum agglutination tests; (3) drug sensitivity test for understanding the serotypes and different resistance pattern of Salmonella by isolates. Serotpying is comprised of 18 serotypes of Salmonella. Out of all isolates, S. Typhimurium had the highest percentage (29 %; 62/215) followed by S.
Enteritidis (27 %; 58/215). Most of the strains tested were resistant to ampicillin (34 %; 72/215) and sulfamethoxazole-trimethoprim (26 %; 55/215). S. Typhimurium serotypes were more resitant to ampicillin (55 %; 34/62). In this study, the presence of class Ⅰ integrons in clinical isolates of Salmonella was detected by PCR. Of 215 Salmonella isolates, 32 carried detectable class Ⅰ integrons. The gene cassettes found almost encoding resistance to β-lactams (blaP1). The results exhibit integrons and antibiotic-resistant gene cassettes of Salmonella of isolates may play an important role in the development and dissemination of multiple antibiotic resistance.
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