Effects of the mycelium from submerged cultured Antrodia camphorata on glucose tolerance and carbohydrate metabolism in type 1 diabetic rat

• Main Authors: Chung-Ying I, 易宗瑛
• Other Authors: Szu-Chuan Shen
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/72477200318378042446

碩士 === 國立屏東科技大學 === 食品科學系所 === 96 === The contents of abstract in this thesis: The purpose of this study is to investigate the effect of the mycelium of submerged cultured Antrodia camphorata in lowing blood sugar and hepatic carbohydrate metabolism. Wistar rats were treated with streptozotocin (STZ) to become induced diabetic rats and applied to the study. Ames test was used to analyze the anti-mutagenic effect for the mycelium of submerged cultured Antrodia camphorata. The acute oral glucose tolerance test (OGTT) results showed that the mycelium of submerged cultured Antrodia camphorata can alleviate the rising of blood sugar in STZ-induced diabetic rats. Especially, the effects in high dose of OGTT (fed with 400 mg/kg of sugar) were more obvious than in low dose of OGTT (200 mg/kg). After 6-week studies, the diabetic rats fed with mycelium of submerged cultured Antrodia camphorata doesn’t show significantly in improvement on glucose tolerance. The rats were sacrificed after 6 weeks. The activities of liver enzymes participating in carbohydrate metabolism were analyzed. The results showed that mycelium of submerged cultured Antrodia camphorata could increase the activities of hexokinase (HKase), phosphofructokinase (PFKase), glucose-6-phosphate dehydrogenas (G6PDHase), but decreased the activities of fructose-1,6-bisphosphatase (F1, 6 BPase) and Glucose-6-phosphatase (G6Pase). It suggests that the mycelium of submerged cultured Antrodia camphorata can promote glucose to go in glycolysis pathway and pentose monophosphate shunt, and reduce the release of glucose from liver. Liver enzyme activities, included HKase, PFKase, G6PDHase, are higher in diabetic rats fed with mycelium of submerged cultured Antrodia camphorata than STZ-induced diabetic rats. On the other hand, the activities of F1, 6BPase, G6Pase of diabetic rats fed with mycelium of submerged cultured Antrodia camphorata are significantly lower than STZ-induced diabetic controls. Salmonella typhimurium TA100 was used for Ames test to analyze the mutagenesis capability of aqueous extract from mycelium of submerged cultured Antrodia camphorata. The results showed that the aqueous extract have wither toxicity nor mutagenesis characteristic in the test concentration of 0.06-1.0 mg/plate. Anti-mutagenetic activity of aqueous extract from mycelium of submerged cultured Antrodia camphorata show inhibitory effect efficiently treated with 4-NQO than treated with B[a]P. In conclusions, the mycelium of submerged cultured Antrodia camphorata can improve the hepatic carbohydrate metabolic enzyme activities in STZ-induced diabetic rats, nevertheless, this effect in OGTT and blood sugar reduction was not significant in our study.