Establishment of culture system and Agrobacterium-mediated gene transformation of 2-acetyl-1-pyrroline synthesis related gene in rice

• Main Authors: Min-Ei Mu, 吳銘益
• Other Authors: Tzau Chi Huang
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/34490781724144348836

碩士 === 國立屏東科技大學 === 生物科技研究所 === 96 === The contents of abstract in this thesis:The purpose of the research was to establish the agrobacterium transformation system of 2-AP (2-acetyl-1-pyrroline) related gene. In the previous research, methylglyoxal(MG) and P5C (1-pyrroline-5-carboxylate) was supposed as the precursor of the 2-AP. In this research, the c-DNA of the gene 1-pyrroline-5 carboxylate synthetase(P5CS) and fructose bis-phosphate aldolase(FBA), extracted form the scented vegetable soybean, was cloned and transferred into the rice seedling via agrobacterium-mediated transformation in order to study the 2-AP biosynthesis. The rice embryogenic-calli culture system was established, and the transformed plant was obtained when the culture system was used as the basic for the agrobacterium transformation. The leaf of the P5CS transgenic plant turned brown was supposed as the toxic effect due to the P5C accumulation, and the FBA transformed calli over-contaminated was explained by the bacterium activation due to the accumulation of the acetyl-coA. The system established was suitable for the agrobacterium transformation of the 2AP synthesis related gene.