| Summary: | 碩士 === 國立高雄師範大學 === 化學系 === 96 === abstract
This research is to analyze the concept of Sulfaguanidine、Sulfani lamide、Sulfacetamide、Sulfadiazine、Sulfathiazole、Sulfamerazine、Sulfamethazine、Sulfamethizole、Sulfameter、Sulfamonomethoxine、Sulfachloropyridazine、Sulfadoxine、Sulfamethoxazole、Sulfisoxazole Sulfadimethoxine、Sulfaquino- xaline、Sulfaphenazole，seventeen sulfonamides in the milk by solid-phase extraction column with high performance liguid chromatography. The experiment discussing (1) the optimal chromatographic evaluaion to simultaneous analyze seve- nteen sulfonamides (2) the optimal condition of solid-phase extraction column，including the time of desorption、the slovent intensity of desorption、the test of absorption capcity etc...(3)the pre-treatment of the real sample、the recycling rate of the real sample and the repro-
ducibility (RSD value) of the real sample.
The experimental result showing the best chromatographic evaluat- ion are :mobile phase A: 0.01M formic acid (pH=2.85),mobile phase B:0.4% formic acid 99.6% ACN,flow rate is 1.0 ml/min,the dectection wave set at 270nm ,Temperature is 28oC , the gradient elution is: 0-2 min: 6% mobile phase B；3-11min: 17.5% mobile phase B:12-33 min :24% mobile phase B. Using the mixing of the sonfonami- des to discussed the best sensitivty of detected methode,can efficientlly sep- arate seventeen sulfonamides ,and also known MIP could rich analy- tes、have selectivity ability.For the linear range is at 0.005~0.1 μg/L，the correlation coefficients are greater than 0.995、and the R.S.D are below 6% and detection limit can get ppb range for the all of sulfonamides.
Finally，the optimal experimental options could be used to detect the residual sulfonamide in the milk,and get good recovery and reproducibility , therefore ,getting up a quick and simple methode for Solid-pahse extraction technique.
|
|---|
