Evaluation on the efficacy of antimicrobial peptide (epinecidin-1) gene therapy against Vibrio vulnificus infection with Zebrafish to a model fish
碩士 === 國立宜蘭大學 === 食品科學系碩士班 === 96 === Organisms can produce antimicrobial materials as the first line of defence to resist microorganism invading and infection. A group of proteins, which are capable of resisting microorganism infection, named antimicrobial peptide (AMP), have been found in plants,...
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| Format: | Others |
| Language: | zh-TW |
| Published: |
2008
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| Online Access: | http://ndltd.ncl.edu.tw/handle/74625144406749878823 |
| Summary: | 碩士 === 國立宜蘭大學 === 食品科學系碩士班 === 96 === Organisms can produce antimicrobial materials as the first line of defence to resist microorganism invading and infection. A group of proteins, which are capable of resisting microorganism infection, named antimicrobial peptide (AMP), have been found in plants, invertebrates and vertebrates. The grouper epinecidin-1 protein with 67 amino acid residues can inhibit Gram-positive and Gram-negative bacteria. Plasmid DNA containing cytomegalovirus (CMV)-promoter-driven EGFP and epinecidin-1 was administered into muscle of Zebrafish (Danio rerio) by varying electroporation containing number of pulses, voltage and plasmid concentration. To demonstrate the expression of EGFP-epinecidin-1 in the muscle at the site of injection, RT-PCR and immunohistochemistry staining techniques were applied. Foreign DNA was detected by polymerase chain reaction in the injected muscles and other tissues. In addition, we also found that EGFP-epinecidin-1 gene was efficiently expressed in vivo against Vibrio vulnificus 204 significantly (p < 0.01). Signal and cytokine regulation demonstrated that CMV-gfp-epi gene transfer could reduce LPS-induced proinflammatory responses in vivo. This technology may substitute the traditionally used chemotherapy agents and antibiotics in the future and reduce risk to human health.
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