Up-regulation of heme oxygenase-1 expression in gingiva after cyclosporine-A treatment

• Main Authors: LAN, Guo-Lun, 藍國倫
• Other Authors: FU, Earl
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/73457603969400785263

碩士 === 國防醫學院 === 牙醫科學研究所 === 96 === Background: Heme oxygenase-1 (HO-1) is a stress-inducible protein confers cytoprotection, but its role in gingiva during the cyclosporine-A (CsA) therapy is unknown. We used in vivo and in vitro models to investigate the expression of mRNA and protein for HO-1 in gingiva upon CsA treatment. Materials & Methods: Twenty-six male Sprague Dawley rats were assigned to two groups after edentulous ridges establishment. Rats in the CsA group received 30 mg/kg/d of CsA for four weeks, whereas control rats received the solvent only. All rats were killed after four weeks and the edentulous gingivae were excised. mRNA and protein expression of HO-1 in gingivae were determined using reverse transcription–polymerase chain reaction (RT–PCR) and immunohistochemistry (IHC), respectively. For the in vitro study, cultured human gingival fibroblasts were harvested after treatment with various concentrations of CsA, and HO-1 mRNA and protein expression were determined using RT–PCR and Western blotting, respectively. Results: Mean of gingival HO-1 mRNA expression was greater in the CsA group than in the control animals (p = 0.076). IHC staining for HO-1protein was significantly greater in the gingivae of CsA-treated rats than in those of the control group. In fibroblast cultures, expression of HO-1 mRNA and protein also significantly increased after CsA treatment. Conclusions: CsA upregulates gingival expression of HO-1, which may exert a cytoprotective effect.