| Summary: | 碩士 === 國防醫學院 === 公共衛生學研究所 === 96 === This cross-sectional study aimed to evaluate the healthy effects of MBOCA exposure and the relationship to oxidative stress damage and genotoxicity and to discuss the modification effect between genetic polymorphism (CYP3A4) and MBOCA exposure.
The study population was recruited from four MBOCA-manufacturing factories in Taiwan. We divided the study population into exposure group(total MBOCA≧20μg/g creatinine) and control group (total MBOCA<20μg/g creatinine) by the total MBOCA concentration of urine and chose 49 persons for each group after matching with gender and age.The personal characteristics, occupational history, and lifestyle were collected by the self-adminstrated questionnaire.
Urine was collected to measure total MBOCA concentration and 8-Hydroxydeoxyguanosine (8-OHdG) as oxidative stress index. We analysed the the mean number of micronucleate cells and CYP3A4 polymorphism in the lymphocyte form blood sample.
The results showed no difference between exposed workers and controls for 8-OHdG but exposed group was significantly higher than control group for the mean number of micronucleate cells. The impact of MBOCA exposure on the high mean number of micronucleate cells was analysed by logistic regression model adjusted for smoking, drinking tea and CYP3A4. The odds ratio was 9.26 (95% CI: 1.83-46.66) in the MBOCA exposure group and the odds ratio was 4.33 (95% CI: 1.20-15.67) in CYP3A4 mutation type.
Our study suggested exposed to MBOCA could induce genotoxicity, and mean number of micronucleate cells was a good indicator to evaluate genotoxicity. Persons who CYP3A4 RS2246709 genotype were mutation type, they were genotoxicity more serious There was not interaction between MBOCA exposure and CYP3A4 polymorphism. We will use another funtional mutation to find the interaction between environment exposure and gene in the future.
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