The Study of the Correlation between Protein Phosphorylation and Ability of Cell Adhesion

• Main Authors: Min-Chih Hsu, 許閔智
• Other Authors: Chyung Ay
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/4pkge5

碩士 === 國立嘉義大學 === 生物機電工程學系研究所 === 96 === This study investigated the relationship between the cell adhesion and activation of protein when the human umbilical vein endothelial cells (Human Umbilical Vein Endothelial Cell Line, ECV304) cultured in a coating of collagen protein and fibronectin of biomedical materials-dimethyl siloxane (Polydimethylsiloxane, PDMS). The electrophoresis force is used to measure the attached ability of ECV304 then driving voltage of electrodes to produce the electrophoresis force meant the adhesive ability of cell. The difference between the adhesive ability of cell and activation of protein is compared under ECV304 cells cultured in a coating of collagen protein and fibronectin on PDMS. Then find out which extracellular matrix (Extracellular Matrix, ECM) cells can provide a more stable growth environment for cell for the collagen protein and fibronectin. The results revealed that when cells cultured in a collagen protein coating on the PDMS which adhesive ability is more instability. The adhesive ability for the second and fifth hours increased and the activation of the focal adhesion kinase (Focal Adhesion Kinase, FAK) also increased for the second and fifth hours. When cells cultured in a coating of the fibronectin attached PDMS which adhesive ability is more stable. It showed that adhesive ability increased as cultured time and FAK activation phenomenon also increased as time. On the activation of protein, the experiment showed the significant relationship between the adhesive ability of cell and FAK activation phenomenon. This study attempts to join three inhibitors, AG18, Genistein and LY294002 to curb Lao Ansuan protein phosphorylation. It shows Genistein on the adhesive ability and the inhibition of protein activation is more effective. However FAK may be an important role on the adhesive ability of cell.