Multiplication of Axillary Buds and In Vitro Flowering Performance via Microshoots in Poinsettia

• Main Authors: Hsiu-Hsia Lin, 林秀霞
• Other Authors: Rong-Show Shen Ph.D.
• Format: Others
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/83204927914787722000

碩士 === 國立嘉義大學 === 農學研究所 === 96 === Three cultivars of commercial Poinsettias (‘Lemon Snow’, ‘Da Vinci’ and ‘Pepride Red’) were prorogated by micropropagated in order to offer a all year around views of commercial production. The present study was probed to pinch, leaf remove and cytokinins to induce the formation of axillary buds by cultural nodes. Results show that if these three cultivars were treated with 100 mg/L of BA, more proliferation rate of axillary buds was observed. In induction and proliferation of axillary buds by nodal sections, when cultivars were treated with BA and coconut water, more number of lateral buds and longer length of shoot were found. When ‘Lemon Snow’ is treated with 3.0mg/L BA with 200 mg/L coconut milk, the result shows better proliferating effects (6.0). In addition, treatments of 1.5mg/L of BA with 150 mg/L coconut milk in‘Pepride Red’have better proliferating effect (7.8). If‘Da Vinci’ is treated 1.5mg/L of BA with 200 mg/L coconut milk, the better proliferating effect (3.9) is found. Adding activated charcoal was a key factor for rate of browning. In vitro rooting from microshoot and defoliation by treatments with various IBA concentrations, the highest in ‘Lemon Snow’ and ‘Pepride Red’ is to treat with 2 mg/L IBA. In Addition, ‘Da Vinci’ has the highest rate for development of roots when this cultivar is treated with 4 mg/L IBA. By utilization of same amount of IBA with various concentrations of STS, ‘Da Vinci’ in treatments of 4 mg/L IBA adding with 0.01 or 0.5 g/L of STS show more numbers/rates of rooting from in vitro microshoot and defoliation. Others of various STS levels with same amount of IBA treatments in different cultivars have no significant differents. Optimal inhibition of defoliation in ‘Pepride Red’ was observed. When ‘Lemon Snow’ is treated with 0.5 or 1.0 g/L of STS with same amount of IBA, the results found that there were significant differents, as comparing to the same cultivar without STS treatment. All of trearments in ‘Da Vinci’ have no significant differents. ‘Pepride Red’ with treatment of retardant PP333 shows best expecting effects in experiment of growth retardant and preservative on the inhabit defoliation from in vitro microshoot. In treatments of growth retardant PP333 and floral preservation, numbers of defoliation in ‘Da Vinci’ have about 2 times more than in cultivar of ‘Lemon Snow.’ By application of various concentrations of IBA with/without 0.005mg/L of STS on the turn color of bracts from in vitro microshoot of Poinsettia, three different cultivars can all turn coloration of bract in the earlier flowering stage. During treatments of various concentrations of STS with same concentration of IBA, results found that low concentration of STS can lead to turn coloration of bract and form flower-bract in earlier stage in these three cultivars. However, there was no significant differents show in high concentration of STS.