Fibrillar Collagen Inhibits Proliferation and Induces Differentiation of Human Placenta Derived Multipotent Cells into Smooth Muscle Cells

• Main Authors: Bo-Min Chou, 周伯珉
• Other Authors: Cheng-Nan Chen
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/46846955114370640472

碩士 === 國立嘉義大學 === 生化科技研究所 === 96 === We isolated placenta derived multipotent cells (PDMCs) from human placental tissues by enzymatic digestion. With RT-PCR and flow cytometric analysis, these PDMCs exhibit many markers, such as Oct-4 and CD166. Immunophenotyping of PDMCs reveals these cells to be positive for many markers common to MSCs SH-2/CD105, CD13, CD29, CD44, and CD166. In the present study, PDMCs grown on fibrillar collagen has showed time-dependent increases in the expression of smooth muscle cell (SMC)-specific markers, including smooth muscle (SM) α-actin, and calponin. Abnormal proliferation of smooth muscle cells (SMCs) is a critical process for the development of vascular disease, including atherosclerosis, vessel renarrowing after successful angioplasty, and graft atherosclerosis after coronary transplantation. Atherosclerosis is emerged by inflammatory metabolic change with lipid accumulation in the artery. The course of atherosclerotic plaque formation, secreted growth factors and cytokines promote the migration and abnormal SMC proliferation is thought to contribute to neointima formation. We demonstrate that SMCs differentiated from PDMCs inhibit cell proliferation on polymerized type I collagen fibrils in vitro. Furthermore, up-regulation of p27 expression may be involved in the modulation of SMC proliferation.