DNA-Grafted Janus Particles

• Main Authors: Ching Hsueh, 薛京
• Other Authors: Wen-tau Juan
• Format: Others
• Language: en_US
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/7kc9d4

碩士 === 國立中央大學 === 物理研究所 === 96 === We developed novel colloidal particles which are composed of a head and tails. The “head” is based on Janus microspheres, amphiphlic particles with two different chemically modified hemispheres. The “tails”are attached by grafting l-DNA onto one of the hemisphere. Both wax-emulsion and gold-evaporation methods are used to generate Janus microspheres. We also test three different biochemical protocols to graft DNA onto silica surface: streptavidin-biotin binding, amine-aldehyde binding and thiol-gold binding. We compared all the methods and found that combining gold-evaporated Janus microspheres with thiol-DNA is an efficient way to fabricate the new class colloidal particles with head-and-tail structures.