Effect of the green tea (-)-epigallocatechin-3-gallate receptor on growth of human MCF7 cells

• Main Authors: Yu-Jung Hu, 胡毓容
• Other Authors: Yung-Hsi Kao
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/36995504432055957716

碩士 === 國立中央大學 === 生命科學研究所 === 96 === LAMR1 is an 295 a.a. non-integrin receptor and has a high affinity for laminin. It was found to enhance the invasion and metastasis of cancer cells. To examine whether any of the specific amino acid domain of LAMR1 protein is responsible for its regulating growth of breast cancer cells, I have stably cloned several lines of MCF7 which contain the full length or four different C-terminus-truncated forms of LAMR1(the respective insert sizes of 200, 150, 100, and 55 a.a.), which were fused with or without Flag protein. MCF7 cells transfected with full length of the LAMR1 or 1-200 a.a. of LAMR1 exhibited increase in the cell numbers more rapidly over the 8-day incubation than the cells transfected with the vehicle. Other transfected MCF7 cells did not grow quickly than did the cells transfected with the vehicle. This suggests that the region of LAMR1 at position 151-200 a.a. is important to the growth of MCF7 cells. Because the LAMR1 is also a receptor for green tea (-)-epigallocatechin-3-gallate (EGCG) and involved in the EGCG inhibition of growth of human lung cancer cells, we like to know whether any of the specific amino acid domain of LAMR1 is responsible for EGCG in regulating growth of breast cancer cells. First, pretreatment of the MCF7 cells with the LAMR1 antiserum could prevent the EGCG-reduced cell numbers. Second, MCF7 cells transfected with full length of LAMR1 were more sensitive to EGCG than were other cells transfected with truncated forms of LAMR1. Results of this study appear to find a functional region of the LAMR1 mediating the effects of EGCG on growth of human breast cancer cells. Further, we can use the signal transducer of the LAMR1 to confirm the eventually function of different C-terminus-truncated forms of LAMR1.