A novel method for detecting microalbuminuria by surface plasmon resonance

• Main Authors: Po-Shen Lin, 林勃伸
• Other Authors: Wen-Yin Chen
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/17888366379943572895

碩士 === 國立中央大學 === 化學工程與材料工程研究所 === 96 === Human serum albumin (HSA) is the most abundant protein in human blood plasma. It is produced in the liver. Albumin comprises about half of the blood serum protein. It is soluble and monomeric. In the healthy kidney, albumin''s size and negative electric charge exclude it from excretion in the glomerulus. But in some diseases including diabetic nephropathy, it can cross the glomerulus and stockpile in the bladder. Depending on the amount of albumin in the urine, a patient may have normal renal function, microalbuminuria, or albuminuria.So urine HSA is an important bio-marker to diagnose nephropathy. Cibacron Blue F3G-A is an anthraquinone dyes which has special affinity for HSA and it`s structure is similar to bilirubin. In this study, Cibacron Blue F3G-A was covalently attached onto PEG monolayer provided with low-fouling property which was established by self-assembled monolayer. The goals of this study used surface plasmon resonance (SPR) to detect HSA and to establish the optimum condition. The results exhibited the chip had high affinity, high capacity and could regeneration in the condition of pH 4. The results also show the pH value can change the HSA’s structure and the structure change is an important factor to raise the affinity constant. The same result also proved by ELISA. In addition, linear response appeared in concentration of HSA in the range of 0.01~0.1mg/ml. The detection limit was 4μg/ml . In other words, SPR biosensor is used as a sensitive technique for direction of diagnosis is powerful.